50  胞内斑点测量

胞内亮点/斑点的测量

1 理论基础

针对感兴趣分子（比如转录本(1)、蛋白(2)、或其它分子）的（免疫）染色研究会在样本中检出很多亮点或斑点。这些亮点就是所感兴趣的分子。对这些亮点的选择和定量对解释科学问题至关重要。

2 流程和实例

说明：示例图片来自Spotiflow (3)。

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References

1.

D. Gyllborg, C. M. Langseth, X. Qian, E. Choi, S. M. Salas, M. M. Hilscher, E. S. Lein, M. Nilsson, Hybridization-based in situ sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue. Nucleic Acids Research 48, e112–e112 (2020).

2.

W. Sheng, C. Zhang, T. M. Mohiuddin, M. Al-Rawe, F. Zeppernick, F. H. Falcone, I. Meinhold-Heerlein, A. F. Hussain, Multiplex immunofluorescence: A powerful tool in cancer immunotherapy. International Journal of Molecular Sciences 24, 3086 (2023).

3.

A. Dominguez Mantes, A. Herrera, I. Khven, A. Schlaeppi, E. Kyriacou, G. Tsissios, E. Skoufa, L. Santangeli, E. Buglakova, E. B. Durmus, S. Manley, A. Kreshuk, D. Arendt, C. Aztekin, J. Lingner, G. La Manno, M. Weigert, Spotiflow: Accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression. Nature Methods 22, 1495–1504 (2025).